Genomics & Proteomics facilities @VIMTA Life Sciences, Hyderabad, India.

Genomics/Proteomics facilities @VIMTA Life Sciences, Hyderabad, India.

VIMTA is offering multidisciplinary contract research and testing services. Recently VIMTA established Advanced Molecular Biology lab with Affymetrix Microarray platform, Proteomics, DNA sequencing, SEQUENOM platform & Custom peptide synthesis facilities with the main intention to offer contract research services to the scientific community with the latest advancements in Molecular Biology.

VIMTA is having the Affymetrix microarray platform which is the most reliable and reproducible in results when compared to cDNA Microarray. VIMTA is offering both the Expression and SNP Genotyping services using Affymetrix platform.

VIMTA work closely with the clients in a professional and confidential manner. VIMTA's client list includes academic and commercial institutions from around the world. Quality in the work and confidentiality in the results is VIMTA's motto.

For more information regarding the services, please mail to: ravi.chilukoti@vimta.com

or

VIMTA LABS LTD, Life Sciences Facility,
Plot No.5, SP Biotech Park,
Genome Valley,
Shameerpet,Hyderabad-500078
Phone (Off): 040-39 84 84 84 Ext: 4302/4215/4207

Human Genome CGH Oligo Microarray Kit

Human Genome CGH Oligo Microarray Kit

Agilent Technologies has introduced its Human Genome CGH Microarray and CGH Analytics software designed to help cancer biologists and cytogeneticists study genomic alterations in cancer. Comparative genomic hybridization (CGH) is used to study the chromosomal changes that frequently occur in cancer....

Human Genome Survey Microarray

Applied Biosystems has announced the release of the Human Genome Survey Microarray V2.0 for use with the Applied Biosystems Expression Array System, a component of Applied Biosystems' comprehensive solution for gene expression analysis that allows researchers to easily move from whole-genome analysis to single gene validation.

Nanotechnology: 'Buckypaper' from coaxial nanotubes

Genomics/Proteomics facilities @VIMTA Life Sciences, Hyderabad, India.

VIMTA is offering multidisciplinary contract research and testing services. Recently VIMTA established Advanced Molecular Biology lab with Affymetrix Microarray platform, Proteomics, DNA sequencing, SEQUENOM platform & Custom peptide synthesis facilities with the main intention to offer contract research services to the scientific community with the latest advancements in Molecular Biology.

VIMTA is having the Affymetrix microarray platform which is the most reliable and reproducible in results when compared to cDNA Microarray. VIMTA is offering both the Expression and SNP Genotyping services using Affymetrix platform.

VIMTA work closely with the clients in a professional and confidential manner. VIMTA's client list includes academic and commercial institutions from around the world. Quality in the work and confidentiality in the results is VIMTA's motto.

Please Contact us:
VIMTA LABS LTD, Life Sciences Facility,
Plot No.5, SP Biotech Park,
Genome Valley,
Shameerpet,Hyderabad-500078
Phone (Off): 040-39 84 84 84 Ext: 4302/4215/4207

For more information regarding the services, please mail to: ravi.chilukoti@vimta.com

BRET Technique: Monitoring modification

Bioluminescence resonance energy transfer (BRET) is a new technique that can be used to detect in situ changes in protein ubiquitylation.

"Ubiquitylation is a reversible post-translational modification that has important roles in processes such as protein degradation and intracellular signalling. Yet, despite a growing interest in ubiquitylation, a tool for studying the dynamics of this process has been lacking. Now though, in Nature Methods, Bouvier and colleagues describe a technique that can be used to detect in situ changes in protein ubiquitylation. ....."

An oncogenic KRAS2 expression signature identified by cross-species gene-expression analysis

An oncogenic KRAS2 expression signature identified by cross-species gene-expression analysis

Interesting article on cross-species gene-expression in NATURE. Using advanced gene targeting methods, generating mouse models of cancer that accurately reproduce the genetic alterations present in human tumors is now relatively straightforward. The challenge is to determine to what extent such models faithfully mimic human disease with respect to the underlying molecular mechanisms that accompany tumor progression. Here we describe a method for comparing mouse models of cancer with human tumors using gene-expression profiling. We applied this method to the analysis of a model of Kras2-mediated lung cancer and found a good relationship to human lung adenocarcinoma, thereby validating the model. Furthermore, we found that whereas a gene-expression signature of KRAS2 activation was not identifiable when analyzing human tumors with known KRAS2 mutation status alone, integrating mouse and human data uncovered a gene-expression signature of KRAS2 mutation in human lung cancer. We confirmed the importance of this signature by gene-expression analysis of short hairpin RNA−mediated inhibition of oncogenic Kras2. These experiments identified both a pattern of gene expression indicative of KRAS2 mutation and potential effectors of oncogenic KRAS2 activity in human cancer. This approach provides a strategy for using genomic analysis of animal models to probe human disease.

A gene highly expressed in tumor cells encodes novel structure proteins

"We isolated several related but distinct cDNA clones encoding novel structure proteins (NSP) when screening a cDNA library. Analysis revealed that these cDNAs and several similar ESTs in the public databases are derived from a single gene of 17 exons that span a minimum of 227-kb region. ...."

Cross-species comparisons of cancer signaling

"Modeling of human cancer in genetically engineered mice and genome-wide measurements of mRNA expression have now been combined to accelerate the search for new molecularly targeted therapies against cancer. Looking forward, we propose a community-wide systematic effort to model and characterize an array of different cancer-related aberrant signaling pathways in several mouse models of cancer...."

PHD Studentship

University of Sussex PHD STUDENTSHIP

"University of Sussex PHD STUDENTSHIP

Genome Damage and Stability Centre

The Genome Damage and Stability Centre is an internationally renowned Institute carrying out research on the response of cells to DNA damage, genome instability and its relationship to human disease. We are seeking motivated individuals to fill the posts and studentships described below. We provide a stimulating and supportive environment and our expertise covers a range of experimental systems. Further information about our research can be obtained from our website at http://www.sussex.ac.uk/gdsc/

PHD STUDENTSHIPS FUNDED FOR 3 YEARS......"

TECHNOLOGY FEATURE

Western blot analysis with quantum dot fluorescence technology: a sensitive and quantitative method for multiplexed proteomics
"Western or immunoblotting analysis of protein expression in cells and tissues has been the major analytical tool for assessing molecular biological functions in basic cell biological research, pathology and drug discovery and development. With the rapid growth of our understanding of gene and protein expression pathways and limited supplies of tissue, there is an ever-increasing need for more sensitive, quantitative methods capable of multiplex detection of proteins or protein states from a single western blot. Quantum Dot Corporation's (QDC) new western blot detection kits harness the high brightness, extreme stability and multiple colors of the Qdot Secondary Antibody Conjugates to quantitatively detect several proteins in a single gel. When used in combination with existing user-defined primary antibodies, the kit combines ease of use with sensitivity to picogram levels of protein using several existing gel imaging systems. ....."

Classic Protocol-Two-dimensional gel electrophoresis of proteins

Two-dimensional gel electrophoresis of proteins

"Two-dimensional polyacrylamide gel electrophoresis (PAGE) is used for separation of complex protein mixtures by the independent parameters of isoelectric point and molecular weight. Isoelectric focusing (IEF) separates proteins in a pH gradient. Each protein is 'focused' because it moves under the influence of the electric field until it reaches its isoelectric point, the pH at which it has no net charge. After IEF in the presence of urea and a nonionic detergent, the IEF gel is equilibrated in sodium dodecyl sulfate (SDS) to prepare the proteins for SDS-PAGE. The method described here1 uses carrier ampholytes to form a pH gradient in a long, thin (1.2-mm) focusing gel composed of a low percentage (2.7%) of acrylamide and containing 9.5 M urea and 2% Nonidet P-40 to maintain protein solubility. After IEF, the gel is briefly equilibrated in SDS and placed directly onto the top edge of a second-dimension slab gel. Because the time between the end of IEF and the start of SDS-PAGE is only a few minutes (thereby minimizing diffusion), the spots are highly resolved and nearly round in shape.
....."